Modeling and Molecular Dynamics of Aquaporin from an Antarctic Pseudomonas sp. Strain AMS3

Aquaporins, also known as water channels, are a large family of transmembrane channel proteins present throughout all life domains and implicated in human disorders. The psychrophilic aquaporin comes to attention because its specialty adaptive ability keep on functioning maintain homeostasis under low temperatures, which have an optimal temperature for growth at about 15ºC or lower. However, studies regarding isolated from Pseudomonas sp. still scattered. Recently, the genome sequence Antarctic strain AMS3 revealed gene encoding putative designated PAqpZ2_AMS3. In this study, structure analysis molecular dynamics (MD) simulation predicted model fully hydrated monomer was embedded lipid bilayer performed different temperatures structural flexibility stability analysis. MD results that could remain stable flexible medium temperatures. addition, important position gating amino acids, Phe36 Asn180 residues were rearranged -5ºC simulation, leading changes column size. information obtained aquaporin, PAqpZ2_AMS3, provides new insights into adaptation protein be useful tool low-temperature industrial applications engineering purposes future.